Researchers have developed a new approach to assess whether a flu vaccine activates the kind of immune cells needed for long-lasting immunity.
Using this technique, the researchers showed that the flu vaccine is capable of eliciting antibodies that protect against a broad range of flu viruses, at least in some people.
Their findings could aid efforts to design an improved flu vaccine that provides protection not only against old influenza viruses but also new ones.
Flu season comes around like clockwork every year, and sooner or later everyone gets infected. The annual flu shot is a key part of public health efforts to control the flu, but the vaccine’s effectiveness is notoriously poor, falling somewhere from 40% to 60% in a typical year.
A growing body of evidence suggests that a history of exposure to influenza virus might undermine the effectiveness of the annual flu vaccine. Partial immunity developed during prior flu seasons—either through natural infection or vaccination—might interfere with the body’s response to a new vaccine, such that vaccination mainly boosts the recognition of prior influenza strains but does little to create the ability to fight new strains.
“Every year, about half of the US adult population gets vaccinated against influenza,” says senior author Ali Ellebedy, assistant professor of pathology and immunology at Washington University in St. Louis.
“It’s necessary for public health, but it’s also incredibly expensive and inefficient. What we need is a one-and-done influenza shot, but we are not there yet. Anything that helps us understand how immunity develops in the context of prior exposures would be important as we try to make a better vaccine.”
Getting the immune system up to date
The key to long-lasting immunity lies in lymph nodes, minuscule organs of the immune system positioned throughout the body. Easy to miss in healthy people, lymph nodes become swollen and tender during an infection as immune cells busily interact and multiply within them.
The first time a person is exposed to a virus—either by infection or vaccination—immune cells capture the virus and bring it to the nearest lymph node. There, the virus is presented to so-called naïve B cells, causing them to mature and start producing antibodies to fight the infection. Once the virus is successfully routed, most of the immune cells that take part in the battle die off, but a few continue circulating in the blood as long-lived memory B cells.
The second time a person is exposed to a virus, memory B cells quickly reactivate and start producing antibodies again, bypassing naive B cells. This rapid response quickly builds protection for people who have been re-infected with the exact same strain of virus, but it’s not ideal for people who have received a vaccine designed to build immunity against a slightly different strain, as in the annual flu vaccine.
“If our influenza vaccine targets memory cells, those cells will respond to the parts of the virus that haven’t changed from previous influenza strains,” Ellebedy says. “Our goal is to get our immune system up to date with the new strains of influenza, which means we want to focus the immune response on the parts of the virus that are different this year.”
Steps toward a universal flu vaccine
To get decades-long immunity against the new strains, the flu strains from the vaccine need to be taken to the lymph nodes, where they can be used to train a new set of naïve B cells and induce long-lived memory B cells specifically tailored to recognize the unique features of the vaccine strains.
To find out what happens inside lymph nodes after influenza vaccination, Ellebedy enlisted the help of coauthors Rachel Presti, associate professor of medicine, and Sharlene Teefey, professor of radiology.
Presti led a team at the Infectious Disease Clinical Research Unit that coordinated the sampling of blood and lymph nodes from healthy volunteers before and after vaccination.
Using ultrasound imaging, Teefey carefully extracted so-called germinal centers that hold immune cells from underarm lymph nodes of eight healthy, young volunteers vaccinated with the 2018-19 quadrivalent influenza vaccine. Scientists had designed that vaccine to protect against four different strains of influenza virus. Researchers extracted the immune cells at one, two, four, and nine weeks after vaccination.
Ellebedy and colleagues analyzed the immune cells in the germinal centers to find the ones that had been activated by vaccination.
In three volunteers, both memory B cells and naïve B cells in the lymph nodes responded to the vaccine strains, indicating that the vaccine had initiated the process of inducing long-lasting immunity against the new strains.
“Our study shows that the influenza vaccine can engage both kinds of cells in the germinal centers, but we still don’t know how often that happens,” Ellebedy says. “But given that influenza vaccine effectiveness hovers around 50%, it probably doesn’t happen as often as we would like. That brings up the importance of promoting strategies to boost the germinal centers as a step toward a universal influenza vaccine.”
The findings appear in the journal Nature. Additional coauthors are from Yale University School of Medicine, Scripps Research Institute, and Washington University in St. Louis.
The National Institute of Allergy and Infectious Diseases of the National Institutes of Health and the Collaborative Influenza Vaccine Innovation Centers supported the work.
