A technique known as magnetic levitation can be used to easily collect and concentrate airborne viruses to help prevent future outbreaks of respiratory disease, researchers report.
Magnetic levitation, or maglev, is the same technology that enables high-speed trains.
“It’s very important to have real-time management and real-time predictions in place for viruses,” says Morteza Mahmoudi, an associate professor in the radiology department and precision health program at Michigan State University. “What we’ve developed is a system that could help us and other stakeholders get more information about the different types of viruses in the air we breathe.”
“This could help identify that an environment is contaminated before a pandemic happens,” says Sepideh Pakpour, an assistant professor of engineering who led the research team at the University of British Columbia Okanagan campus.
In addition to serving as an early-warning system, the team’s new technique also could help health officials and epidemiologists better track and trace exposure to airborne viruses in public settings.
Maglev tech separates airborne viruses
The researchers first started this project applying magnetic levitation to respiratory viruses in 2018 with support from the Walsh Foundation and the New Frontiers in Research Fund. Almost half of lower respiratory tract infections are caused by viruses that people breathe in while indoors, the researchers wrote in their report.
But when the coronavirus pandemic started and as they learned that it was caused by an airborne virus, they knew they had to redouble their efforts. The team used a deactivated version of the coronavirus responsible for COVID-19 in their proof-of-concept report, along with H1N1 influenza and a virus that infects bacteria known as bacteriophage MS2.
The system first collects air samples, then injects the sample into a fluid where maglev separates viruses from other particles. The isolated and purified viral contents are then passed along to other standard analytical techniques for identification in a matter of minutes. The approach is so straightforward that it could be used by nonexperts in a variety of settings, such as clinics and airports, the researchers say.
The team is taking the first steps toward commercializing its technology while working to improve it at the same time.
Although downstream techniques can identify which viruses are in a sample, one of the team’s future goals is refining the maglev step to distinguish between different viruses on its own. The researchers also are working to heighten their technique’s sensitivity and detect viruses in air at lower concentrations.
Still, the team is excited by what it was able to accomplish in its initial work and by what it may enable other researchers to do.
“Using maglev for disease detection and purifying viruses is brand new, and it could open up applications in many different fields,” Mahmoudi says. “This opens up a fundamentally new direction in analytical biochemistry.”
From floating trains to virus detection
Magnetic levitation, as its name suggests, uses magnets to counteract the downward pull of gravity. Maglev trains float above their tracks and, unencumbered by that contact friction, can achieve speeds upwards of 200 miles per hour. While maglev trains have been around for decades, the use of magnetic levitation in biology is more recent.
For example, it was only within the last decade that Stanford researchers showed that living cells could be magnetically levitated in liquid mixtures or solutions. They then used the technique to show that various cell types—yeast, bacteria, healthy human cells, and cancer cells—could be separated by their density.
More recently, Pakpour and Mahmoudi have collaborated to show that maglev can be applied to proteins in blood plasma to look for indicators of opioid use and multiple sclerosis. That convinced them maglev also should work with viruses.
“If you look at the structure of viruses, they’re mostly proteins, and we knew we could levitate proteins,” Pakpour says. “So I knew this should work, but I was still surprised when it did.”
“It was very challenging, especially at the start,” Mahmoudi says. “Conventional maglev is not capable of collecting submicron viruses, but we made several modifications and were able to fine-tune the system.”
One of the challenges was that the liquids used in conventional maglev could damage or destroy viruses. The team had to find new solutions that had desirable magnetic properties and were compatible with their targets.
The challenges were exacerbated by the pandemic, but the emergence of COVID-19 showed the team how important its work was—and how resilient its members were.
“There were so many obstacles, even just getting samples to each other,” Pakpour says. “But we pushed forward. And, if we won that battle, I think we can win any other.”
The study is published in the journal ACS Nano.
Source: Matt Davenport for Michigan State
