A new sensor could help advance treatment for spinal cord disease and injury, researchers report.
Implantable technologies have significantly improved our ability to study and even modulate the activity of neurons in the brain, but neurons in the spinal cord are harder to study in action.
“If we understood exactly how neurons in the spinal cord process sensation and control movement, we could develop better treatments for spinal cord disease and injury,” says Yu Wu, a research scientist at Rice University and lead author of the study published in Cell Reports.
“We developed a tiny sensor, spinalNET, that records the electrical activity of spinal neurons as the subject performs normal activity without any restraint. Being able to extract such knowledge is a first but important step to develop cures for millions of people suffering from spinal cord diseases.”
“Up until now, the spinal cord has been more or less a black box.”
According to the study, the sensor was used to record neuronal activity in the spinal cord of freely moving mice for prolonged periods and with great resolution, even tracking the same neuron over multiple days.
“Up until now, the spinal cord has been more or less a black box,” says corresponding author Lan Luan, an associate professor of electrical and computer engineering. “The issue is that the spinal cord moves so much during normal activity. Every time you turn your head or bend over, spinal neurons are also moving.”
During such movements, rigid sensors implanted in the spinal cord inevitably disturb or even damage the fragile tissue. SpinalNET, however, is over a hundred times smaller than the width of a hair, which makes it extremely soft and flexible—nearly as soft as the neural tissue itself.
“This flexibility gives it the stability and biocompatibility we need to safely record spinal neurons during spinal cord movements,” says corresponding author Chong Xie, an associate professor of electrical and computer engineering and bioengineering. “With spinalNET, we were able to get low-noise signals from hundreds of neurons.”
The spinal cord plays a critical role in controlling movement and other vital functions, and the ability to record spinal neurons with fine-grained spatial and temporal resolution during unrestrained motion offers a window into the mechanisms that make this possible.
Using spinalNET allowed researchers to determine that the spinal neurons in the central pattern generator—the neuronal circuit that can produce rhythmic motor patterns such as walking in the absence of specific timing information—seem to be involved with a lot more than rhythmic movement.
“Some of them are strongly correlated with leg movement, but surprisingly, a lot of neurons have no obvious correlation with movement,” Wu says. “This indicates that the spinal circuit controlling rhythmic movement is more complicated than we thought.”
The researchers say they hope to help unravel some of this complexity in future research, tackling questions such as the difference between how spinal neurons process reflex motion, getting startled, for instance, versus volitional action.
“In addition to scientific insight, we believe that as the technology evolves, it has great potential as a medical device for people with spinal cord neurological disorders and injury,” Luan says.
The National Institutes of Health, the Salk Institute, the Mary K. Chapman Foundation, and Rice supported the work.
The content in this press release is solely the responsibility of the authors and does not necessarily represent the official views of the funders.
Source: Rice University
