Typically characterized as poisonous, corrosive, and smelling of rotten eggs, hydrogen sulfide may help protect aging brain cells against Alzheimer’s disease, according to new experiments with mice.
The discovery of the biochemical reactions that make this possible opens doors to the development of new drugs to combat neurodegenerative disease.
“Our new data firmly link aging, neurodegeneration, and cell signaling using hydrogen sulfide and other gaseous molecules within the cell,” says Bindu Paul, faculty research instructor in neuroscience in the neuroscience department at the Johns Hopkins University School of Medicine and lead corresponding author of the study in the Proceedings of the National Academy of Sciences.
The human body naturally creates small amounts of hydrogen sulfide to help regulate functions throughout the body, from cell metabolism to blood vessel dilation. The rapidly burgeoning field of gasotransmission shows that gases are major cellular messenger molecules, with particular importance in the brain.
However, unlike conventional neurotransmitters, gases can’t be stored in vesicles. Thus, gases act through very different mechanisms to rapidly facilitate cellular messaging.
Cognitive and motor function improvements
In the case of hydrogen sulfide, this entails the modification of target proteins by a process called chemical sulfhydration, which modulates their activity, says Solomon Snyder, professor of neuroscience and co-corresponding author on the study.
Studies using a new method have shown that sulfhydration levels in the brain decrease with age, a trend that is amplified in patients with Alzheimer’s disease.
“Here, using the same method, we now confirm a decrease in sulfhydration in the AD brain,” says collaborator Milos Filipovic, principal investigator at Leibniz-Institut für Analytische Wissenschaften—ISAS.
For the current research, scientists studied mice genetically engineered to mimic human Alzheimer’s disease. They injected the mice with a hydrogen sulfide-carrying compound called NaGYY, developed by collaborators at the University of Exeter in the UK, which slowly releases the passenger hydrogen sulfide molecules while traveling throughout the body. The researchers then tested the mice for changes in memory and motor function over a 12-week period.
Behavioral tests on the mice showed that hydrogen sulfide improved cognitive and motor function by 50% compared with mice that did not receive the injections of NaGYY. Treated mice could better remember the locations of platform exits and appeared more physically active than their untreated counterparts with simulated Alzheimer’s disease.
The results show that introducing hydrogen sulfide can reverse the behavioral outcomes of Alzheimer’s disease, but the researchers wanted to investigate how the brain chemically reacted to the gaseous molecule.
Reversing aspects of Alzheimer’s
A series of biochemical experiments revealed a change to a common enzyme called glycogen synthase β (GSK3β). In the presence of healthy levels of hydrogen sulfide, GSK3β typically acts as a signaling molecule, adding chemical markers to other proteins and altering their function.
However, the researchers observed that in the absence of hydrogen sulfide, GSK3β is over-attracted to another protein in the brain called tau.
When GSK3β interacts with tau, tau changes into a form that tangles and clumps inside nerve cells. As tau clumps grow, the tangled proteins block communication between nerves, eventually causing them to die. This leads to the deterioration and eventual loss of cognition, memory and motor function that is characteristic of Alzheimer’s disease.
“Understanding the cascade of events is important to designing therapies that can block this interaction like hydrogen sulfide is able to do,” says Daniel Giovinazzo, an MD/PhD student and first author of the study.
Until recently, researchers lacked the pharmacological tools to mimic how the body slowly makes tiny quantities of hydrogen sulfide inside cells.
“The compound used in this study does just that and shows by correcting brain levels of hydrogen sulfide, we could successfully reverse some aspects of Alzheimer’s disease,” says study collaborator Matt Whiteman, professor of experimental therapeutics at the University of Exeter Medical School.
The researchers plan to continue studying how sulfur groups interact with GSK3β and other proteins involved in the pathogenesis of Alzheimer’s disease in other cell and organ systems and also to test new hydrogen sulfide delivery molecules as part of their ongoing venture.
Additional coauthors are from the Leibniz-Institut für Analytische Wissenschaften—ISAS and the Johns Hopkins University School of Medicine.
Funding came from the US Public Health Service Grant, the American Heart Association, the Allen Initiative in Brain Health and Cognitive Impairment, the Medical Research Council of the United Kingdom, the Brian Ridge Scholarship, and the European Research Council. The Johns Hopkins researchers declare no competing financial interests.
Source: Johns Hopkins University
